Influence of oxygen availability on expression of glutaminolysis genes in human colon cancer cells

Abstract Introduction Glutaminolysis, beside glycolysis, is a key metabolic pathway of cancer cell that provides energy and substrates for the synthesis nucleic acids, proteins, lipids. The mediated by both mitochondrial cytosolic enzymes. Neither expression glutaminolysis enzymes in colon cells nor influence various oxygen concentrations on their has been studied so far. Objectives aim study was to determine compare mRNA involved at levels human primary (SW480) metastatic (SW620) cultured 1% O 2 (hypoxia), 10% (tissue normoxia), 21% (atmospheric normoxia). Methods Cell viability determined Trypan Blue exclusion (TB) Thiazolyl Tetrazolium Bromide (MTT). HIF1α, GLUT1, GLS1, AST1, AST2, ACL, PC GC1, GC2 levelwas RT-qPCR. Results. Correlation between increasing concentration count not observed. In lines number viable lowest oxygen. enzyme profile proteins varied depending pressure type lines. summary, our findings suggest differences adaptation availability vivo cells.